910 research outputs found

    A reduced-reference perceptual image and video quality metric based on edge preservation

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    In image and video compression and transmission, it is important to rely on an objective image/video quality metric which accurately represents the subjective quality of processed images and video sequences. In some scenarios, it is also important to evaluate the quality of the received video sequence with minimal reference to the transmitted one. For instance, for quality improvement of video transmission through closed-loop optimisation, the video quality measure can be evaluated at the receiver and provided as feedback information to the system controller. The original image/video sequence-prior to compression and transmission-is not usually available at the receiver side, and it is important to rely at the receiver side on an objective video quality metric that does not need reference or needs minimal reference to the original video sequence. The observation that the human eye is very sensitive to edge and contour information of an image underpins the proposal of our reduced reference (RR) quality metric, which compares edge information between the distorted and the original image. Results highlight that the metric correlates well with subjective observations, also in comparison with commonly used full-reference metrics and with a state-of-the-art RR metric. © 2012 Martini et al

    The Dynactin Complex Enhances the Speed of Microtubule-Dependent Motions of Adenovirus Both Towards and Away from the Nucleus

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    Unlike transport vesicles or organelles, human adenovirus (HAdV) directly binds to the microtubule minus end-directed motor dynein for transport to the nucleus. The dynein cofactor dynactin enhances nuclear transport of HAdV and boosts infection. To determine if dynactin has a specific role in cytoplasmic trafficking of incoming HAdV on microtubules, we used live cell spinning disc confocal microscopy at 25 Hz acquisition frequency and automated tracking of single virus particles at 20–50 nm spatial resolution. Computational dissection by machine-learning algorithms extracted specific motion patterns of viral trajectories. We found that unperturbed cells supported two kinds of microtubule-dependent motions, directed motions (DM) and fast drifts (FD). DM had speeds of 0.2 to 2 μm/s and run lengths of 0.4 up to 7 μm, while FD were slower and less extensive at 0.02 to 0.4 μm/s and 0.05 to 2.5 μm. Dynactin interference by overexpression of p50/dynamitin or a coiled-coil domain of p150/Glued reduced the speeds and amounts of both center- and periphery-directed DM but not FD, and inhibited infection. These results indicate that dynactin enhances adenovirus infection by increasing the speed and efficiency of dynein-mediated virus motion to the nucleus, and, surprisingly, also supports a hereto unknown motor activity for virus transport to the cell periphery

    Generalized Pickands constants and stationary max-stable processes

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    Pickands constants play a crucial role in the asymptotic theory of Gaussian processes. They are commonly defined as the limits of a sequence of expectations involving fractional Brownian motions and, as such, their exact value is often unknown. Recently, Dieker and Yakir (Bernoulli, 20(3), 1600–1619, 2014) derived a novel representation of Pickands constant as a simple expected value that does not involve a limit operation. In this paper we show that the notion of Pickands constants and their corresponding Dieker–Yakir representations can be extended to a large class of stochastic processes, including general Gaussian and Lévy processes. We furthermore develop a link to extreme value theory and show that Pickands-type constants coincide with certain constants arising in the study of max-stable processes with mixed moving maxima representations

    Intra-oral compartment pressures: a biofunctional model and experimental measurements under different conditions of posture

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    Oral posture is considered to have a major influence on the development and reoccurrence of malocclusion. A biofunctional model was tested with the null hypotheses that (1) there are no significant differences between pressures during different oral functions and (2) between pressure measurements in different oral compartments in order to substantiate various postural conditions at rest by intra-oral pressure dynamics. Atmospheric pressure monitoring was simultaneously carried out with a digital manometer in the vestibular inter-occlusal space (IOS) and at the palatal vault (sub-palatal space, SPS). Twenty subjects with normal occlusion were evaluated during the open-mouth condition (OC), gently closed lips (semi-open compartment condition, SC), with closed compartments after the generation of a negative pressure (CCN) and swallowing (SW). Pressure curve characteristics were compared between the different measurement phases (OC, SC, CCN, SW) as well as between the two compartments (IOS, SPS) using analysis of variance and Wilcoxon matched-pairs tests adopting a significance level of α = 0.05. Both null hypotheses were rejected. Average pressures (IOS, SPS) in the experimental phases were 0.0, −0.08 (OC); −0.16, −1.0 (SC); −48.79, −81.86 (CCN); and −29.25, −62.51 (SW) mbar. CCN plateau and peak characteristics significantly differed between the two compartments SPS and IOS. These results indicate the formation of two different intra-oral functional anatomical compartments which provide a deeper understanding of orofacial biofunctions and explain previous observations of negative intra-oral pressures at rest

    Psychophysiology-based QoE assessment : a survey

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    We present a survey of psychophysiology-based assessment for quality of experience (QoE) in advanced multimedia technologies. We provide a classification of methods relevant to QoE and describe related psychological processes, experimental design considerations, and signal analysis techniques. We summarize multimodal techniques and discuss several important aspects of psychophysiology-based QoE assessment, including the synergies with psychophysical assessment and the need for standardized experimental design. This survey is not considered to be exhaustive but serves as a guideline for those interested to further explore this emerging field of research

    Phylogeny of the Sepia pharaonis species complex (Cephalopoda: Sepiida) based on analyses of mitochondrial and nuclear DNA sequence data

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    The pharaoh cuttlefish, Sepia pharaonis Ehrenberg, 1831, is a commercially fished species found from Japan to East Africa. Previous morphological and genetic work (the latter based on the 16S rRNA mitochondrial gene) suggested that S. pharaonis is a species complex, but relationships within the complex remained unresolved. To clarify these relationships, we have sequenced an additional mitochondrial gene region (cytochrome oxidase subunit I) and a nuclear gene region (rhodopsin) from over 50 specimens from throughout the range of S. pharaonis. We have also added sequence data from two specimens of Sepia ramani Neethiselvan, 2001, collected in southeastern India. Sepia ramani is a species that is morphologically very similar to S. pharaonis, and there is some question regarding its status as a distinct species. Phylogenetic analyses of a dataset comprising all three-gene regions revealed a monophyletic S. pharaonis complex consisting of a western Indian Ocean clade, a northeastern Australia clade, a Persian Gulf/Arabian Sea (‘Iranian’) clade, a western Pacific clade and a central Indian Ocean clade. Relationships among these clades remain somewhat poorly supported except for a clade comprising the Iranian clade, the western Pacific clade and the central Indian Ocean clade. One S. pharaonis specimen was collected in the Arabian Sea, but was found to be a member of the western Indian Ocean clade, suggesting that gene flow between these regions has either occurred recently or is ongoing. Both specimens of S. ramani are members of the S. pharaonis complex, but their mtDNA haplotypes are not closely related – one is a member of the central Indian Ocean clade, while the other is rather distantly related to the northeastern Australia clade. We suggest that ‘S. pharaonis’ may consist of several species, but morphological work is needed to clarify species-level taxonomy within this complex

    Metabolic control of tobacco pollination by sugars and invertases

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    Pollination in flowering plants is initiated by germination of pollen grains on stigmas followed by fast growth of pollen tubes representing highly energy-consuming processes. The symplastic isolation of pollen grains and tubes requires import of Suc available in the apoplast. We show that the functional coupling of Suc cleavage by invertases and uptake of the released hexoses by monosaccharide transporters are critical for pollination in tobacco (Nicotiana tabacum). Transcript profiling, in situ hybridization, and immunolocalization of extracellular invertases and two monosaccharide transporters in vitro and in vivo support the functional coupling in supplying carbohydrates for pollen germination and tube growth evidenced by spatiotemporally coordinated expression. Detection of vacuolar invertases in maternal tissues by these approaches revealed metabolic cross talk between male and female tissues and supported the requirement for carbohydrate supply in transmitting tissue during pollination. Tissue-specific expression of an invertase inhibitor and addition of the chemical invertase inhibitor miglitol strongly reduced extracellular invertase activity and impaired pollen germination. Measurements of (competitive) uptake of labeled sugars identified two import pathways for exogenously available Suc into the germinating pollen operating in parallel: direct Suc uptake and via the hexoses after cleavage by extracellular invertase. Reduction of extracellular invertase activity in pollen decreases Suc uptake and severely compromises pollen germination. We further demonstrate that Glc as sole carbon source is sufficient for pollen germination, whereas Suc is supporting tube growth, revealing an important regulatory role of both the invertase substrate and products contributing to a potential metabolic and signaling-based multilayer regulation of pollination by carbohydrates.Peer Reviewe

    The interface between the stellar wind and interstellar medium around R Cassiopeiae revealed by far-infrared imaging

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    The circumstellar dust shells of intermediate initial-mass (about 1 to 8 solar masses) evolved stars are generated by copious mass loss during the asymptotic giant branch phase. The density structure of their circumstellar shell is the direct evidence of mass loss processes, from which we can investigate the nature of mass loss. We used the AKARI Infrared Astronomy Satellite and the Spitzer Space Telescope to obtain the surface brightness maps of an evolved star R Cas at far-infrared wavelengths, since the temperature of dust decreases as the distance from the star increases and one needs to probe dust at lower temperatures, i.e., at longer wavelengths. The observed shell structure and the star's known proper motion suggest that the structure represents the interface regions between the dusty wind and the interstellar medium. The deconvolved structures are fitted with the analytic bow shock structure to determine the inclination angle of the bow shock cone. Our data show that (1) the bow shock cone of 1 - 5 x 10^-5 solar masses (dust mass) is inclined at 68 degrees with respect to the plane of the sky, and (2) the dust temperature in the bow shock cone is raised to more than 20 K by collisional shock interaction in addition to the ambient interstellar radiation field. By comparison between the apex vector of the bow shock and space motion vector of the star we infer that there is a flow of interstellar medium local to R Cas whose flow velocity is at least 55.6 km/s, consistent with an environment conducive to dust heating by shock interactions.Comment: 7 pages, 2 figures, accepted for publication in Astronomy and Astrophysic

    Absolute Flux Calibration of the IRAC Instrument on the Spitzer Space Telescope using Hubble Space Telescope Flux Standards

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    The absolute flux calibration of the James Webb Space Telescope will be based on a set of stars observed by the Hubble and Spitzer Space Telescopes. In order to cross-calibrate the two facilities, several A, G, and white dwarf (WD) stars are observed with both Spitzer and Hubble and are the prototypes for a set of JWST calibration standards. The flux calibration constants for the four Spitzer IRAC bands 1-4 are derived from these stars and are 2.3, 1.9, 2.0, and 0.5% lower than the official cold-mission IRAC calibration of Reach et al. (2005), i.e. in agreement within their estimated errors of ~2%. The causes of these differences lie primarily in the IRAC data reduction and secondarily in the SEDs of our standard stars. The independent IRAC 8 micron band-4 fluxes of Rieke et al. (2008) are about 1.5 +/- 2% higher than those of Reach et al. and are also in agreement with our 8 micron result.Comment: 16 pages, 6 figure
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